Antiviral therapy in shrimp through plant virus VLP containing VP28 dsRNA against WSSV

• Santiago Ramos-Carreño,
• Ivone Giffard-Mena,
• Jose N. Zamudio-Ocadiz,
• Alfredo Nuñez-Rivera,
• Ricardo Valencia-Yañez,
• Jaime Ruiz-Garcia,
• Maria Teresa Viana and
• Ruben D. Cadena-Nava

Beilstein J. Org. Chem. 2021, 17, 1360–1373, doi:10.3762/bjoc.17.95

• applied to pathogen control in shrimp and also other organisms, widening the application window in nanomedicine. Keywords: antiviral therapy; CCMV; oral administration; P. vannamei; plant VLPs; RNAi; VP28; white spot syndrome virus; Introduction The white spot syndrome virus (WSSV) is recognized as one

• electron microscope equipped with a digital camera operated at 200 keV. The size of the VLPs was measured using the ImageJ (U.S. NIH) software from digital recorded TEM images. Shrimp and rearing conditions. P. vannamei postlarvae (PL) were grown in 2,500 L circular tanks containing seawater (34 ppt

• efficacy in P. vannamei against WSSV infection. Experimental results indicate that a minimum dose of 0.5 µg/shrimp is enough to protect up to 65% of the population against the virus. The maximum dose used in the present work was 3.0 µg/shrimp with 95% protection at 504 hpi (21 days). These evaluated doses